DNA goes DIY
Looking for an affordable way to research DNA? Well now a $512 model, brought to light thanks to a Kickstarter campaign, can be yours. The machine is called OpenPCR.
“The Polymerase Chain Reaction, or PCR, is a method of replicating DNA. It is capable of taking a small amount of DNA, or even a single molecule, and amplifying (copying) a specific region exponentially, such that once the reaction is finished, there may exist up to 230 copies of each starting molecule (do the math — that’s more than a billion!).This is important because DNA of interest often exists in quantities too small to detect, or may be mixed in with other DNA. For example, an accurate test for HIV must be able to detect a single virus particle in 50,000 cells. PCR is able to do this by targeting a small region of DNA that is specific to the HIV virus. If the virus exists in a sample, amplification will occur which can be easily detected. If no virus is present, no amplification will occur.The specific region of targeted DNA is determined by how the reaction is setup, based on the specific “PCR primers” added to the reaction mixture. Virtually any sequence of DNA can be targeted.”
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